Primer
pF_fbp-fwd

Part:BBa_K4047002:Design

Designed by: An Nguyen, Avery Imes   Group: iGEM21_MiamiU_OH   (2021-09-03)


primerF_fbp-foward


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 20
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 20
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 20
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 20
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 20
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This primer was created using the information detailed below within the NEBuilder free online tool located at https://nebuilder.neb.com/#!/


Source

Generated from a combination of factors. Overlap sequence sourced from pAM2991. https://www.addgene.org/40248/

Annealing sequence sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for fructose-1,6-bisphosphatase class I, at the locus tag Synpcc7942_2335. https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=2402921&to=2403955&report=fasta&strand=2

References